complex target interactions — Posts

Viewing a Cascade

In 2014, three labs completed structures of different versions of the Cascade complex, in three papers published at the same time – including one led by Sabin Mulepati, a then-PhD student in the Bailey lab. Sabin used x-ray crystallography to determine the structure of Cascade bound to a single strand of target DNA that matched the Cascade crRNA, to a resolution of 3 angstroms.

Sarah StuderOctober 4, 2021paper, type i paper, type i, cascade structure, complex target interactions

What makes a match? For CRISPR type III-B, it depends on location

CRISPR is used by some bacteria to target and destroy DNA and RNA from invading viruses and other sources - but how do they keep from damaging their own DNA?

A recent paper from our lab shows how one type of CRISPR system distinguishes between its own DNA and others.

Sarah StuderOctober 30, 2019paper, type iii paper, type iii, what makes a match, complex target interactions